Active Air Sampling Procedure

Analyze the microbial quality of air by sampling 100 litres of air at each of the locations mentioned in annexure i using active air sampler carry the pre incubated scda plates and sterile ss head in a ss container to the sampling location.

Active air sampling procedure.

Procedure for air sampling in pharmaceuticals 1 prepare scda medium and aseptically pour approximately 15 20 ml of sterile molten cooled 40 c scda agar into sterile 90 mm petri plates 2 allow solidifying the plates at room temp after solidification label all the plates with the name of media preparation batch no. Procedure the microbial environmental conditions of unit ii shall be monitored by settle plate method or air sampling at the locations. And date of preparation. Active there are two primary methods for microbial air sampling.

In active monitoring a microbial air sampler is used to force air into or onto its collection medium e g petri dish with nutrient agar based test media over a specified period of time. Airborne gases and vapours are collected by a physical process such as diffusion through a static air layer or permeation through a membrane. This procedure is applicable for the deployment of pas in urban suburban rural and remote areas. Active and passive monitoring.

The objective of this procedure is to describe the management of air passive samplers pas for sampling pollutants including the main steps for assembling and disassembling as well as maintenance of the passive air samplers. 1 impingers impingers use a liquid medium for particle collection. As i mentioned above in active air sampling we determine bioburden in 1 cubic meter area and we sample 1000 liters of air by air sampler while in passive air sampling we determine that how much microbes settle in 90 mm diameter surface of any equipment exposed in controlled area. In case the active air sampler is out of order then settle plate method can be used as alternative for monitoring the microbiological quality of air.

The collected culture can then be incubated and. Active sampling involves the use of an air sampling pump to actively pull air through a collection device such as a filter.